Laboratory Instruments
Agilent 1100 HPLC System
System Includes:
- Solvent Tray
- G1322 / G1329 Degasser
- G1312 Binary Pump
- G1329 Auto Sampler
- G1330 ALS Therm
- G1365B MWD
HPLC relies on the interaction of sample components with a stationary phase (typically a solid or a liquid packed in a column) and a mobile phase (a solvent or a mixture of solvents) under high pressure.
Column: Contains the stationary phase where separation occurs.
Pump: Generates and maintains high pressure required for solvent flow through the column.
Injector: Introduces the sample into the mobile phase stream entering the column.
Detector: Monitors the eluent leaving the column for analyte detection.
Data system: Collects, analyzes, and presents chromatographic data.
A sample is injected into the mobile phase stream, which carries it through the column.
Components in the sample interact differently with the stationary phase, leading to separation based on their properties (e.g., size, polarity, charge).
Eluted components are detected by the detector, which generates a signal proportional to the concentration of each component.
Pharmaceuticals: Analysis of drug purity, stability, and formulation.
Environmental: Detection of pollutants in water and air.
Biological: Separation of proteins, nucleic acids, and other biomolecules.
Food and Beverage: Analysis of additives, contaminants, and nutrients.
High resolution and sensitivity.
Capability to handle complex mixtures.
Versatility in terms of stationary phase and detection methods.
Reversed-phase HPLC: Stationary phase is non-polar, useful for separating hydrophobic compounds.
Normal-phase HPLC: Stationary phase is polar, useful for separating polar compounds.
Size-exclusion HPLC: Separates molecules based on their size.